Thursday, March 23, 2006

America Needs You

Never have I been so happy that the Indian Nations are exempt from state and federal laws.
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Tuesday, March 21, 2006

tense, anxious and annoyed

alternatively ta^2.
tense because I have to start writing a manuscript and I have literally got five lines in an outline format. I have started this paper twice before, but neither of those scribblings fits in with what my boss thinks we should focus on.
anxious because of the above, but also because an abstract is due in a couple of weeks for a conference in july in Scotland. It would be great to go to the conference, but the plane tix alone are 900 USD just to get to London. Then there is the train and the lodging and all that other stuff. I haven't been to a conference with this boss, I don't know how much she will cover, there is a travel stipend but with conversion it is only about 440 USD. Eek, and I owe Moms $600 for the tix to TX (also in July - hah!) I hate it when I get anxious about money, but let's face it, it's grad school. I managed to get rid of most of my debts while I was working - never managed to save very much but after 5.5 years of grad school have managed to dig that hole again. I am just not sure when I am going to have $1000 to drop on plane and train tix to Scotland and take care of all the other things I want to do. Of course, self restraint would work here, but really, self restraint and moderation are sooo over rated.
Annoyed because in preparation of manuscript writing I have been online at PubMed all day, making sure I am up to date with the literature. I found an absolutely fabulous (or so it would seem) article from Journal of Applied Physiology, which according to PubMed is published. But I can't get my grubby little hands on it! Not even through my jacked H*rvard login. I can get to the journal, but according to the search engine on the journal's website, the article simply does not exist. It is really really annoying when PubMed is more on the ball than the journal doing the actual publishing.
Good Lord, yoga can't come too soon today. Only one hour and fourteen minutes till relaxation. Until then, I wage war against publishing sites that do not readily yield their articles so that I may expand my brain ever so minutely much more.
Ciao

Thursday, March 16, 2006

bad Bad BAD graduate student

OK, I just spent 30 minutes with the Tasmanian Devil Undergrad (the one that leaves a wake of destruction where ever he goes) arguing about affinity purifications. He has NO experience in the lab. He has little experience in coupling chemistry. He does not believe in enzymes. He does not know the pKa of water. And yet, he argues with me about everything.
I know my stuff. I know how to do affinity purification. I have been doing affinity purification in some form or another for close to 12 years. I am good at it. I also know my way around coupling chemistry, since, you know, I was a tech doing synthesis for a couple of years before grad school. I also know my way around some enzymes since I have, you know, an B.S. in biochemistry from a swanky school and 5.5 years completed in my quest for the Ph.D. in biophysics from a swanky biochemistry graduate program and the last three of those years have been spent in the lab of a very reputable and highly esteemed enzymologist. AND, I know the pKa of WATER!!!
And yet he argues. And argues. And asks hazy, infuriatingly vague questions.
And my group meeting for the Stoopid group is not done yet. I don't even know what I want to talk about yet.

Fuck.

Ciao

Sunday, March 05, 2006

second post from Houston

This is the second post from Houston, since I just lost the first one I composed. Blasted Blogger!! this will learn me to save every now and again.
Anyway, we are about to head out to
Body Worlds, which should tickle my macabre bone just fine.
There was a bunch of stuff in here about being a yankee and ten gallon hats and the like, but I really can't be bothered with recreting it right now. The birds are singing, the Wee One is nursing with a grunt and a snort, Ginger the dog lies next to me on the couch, farting up a storm. Those are the sights, sounds and smells of Houston right now.
Breakfast was breakfast tacos. Must remember this. Fantabulous. I had three.
Last night, after navigating the traffic in town for The Rodeo, we headed over to a gathering of Big Papa's family.
More later - gots to go...
ciao

Thursday, March 02, 2006

Really f-ing disgusting

The Chinese are trying to improve their image in light of their hosting the Olympics in a couple of years:
Beijing puts the kibosh on spitting
but I don't think this is going to help.
Dine in or take out?

Wednesday, March 01, 2006

Case in point

Motivation. Ah motivation. I had such high hopes for all the work I was going to complete last night after yoga, not to mention finishing my blog entry, and some how, none and I mean none of it happened.
Last night sucked and I think it affected my motivation. No man, no dog. The absence of both was what led me to originally think that I might get my work done, late into the evening. But when I got home after yoga, to have a quick bite and set the VCR to capture Boston Legal (screw American Idol, I want my House), I got all depressed that Halley was not there to greet me. It was not as prominent the night before, since I went to TSB's for the night. But last night, I was home, alone, and frankly a bit lost. I miss my dog. I miss griping about walking her at 11 PM in February in 4 degree weather. I miss her little paws raking at the covers, demanding I raise them so that she may burrow deep to the warmest part. I miss her sleepy eyes and her whole body shakes that can throw her off balance and after 9 years she still gets surprised by that. I even miss having to think that I have to pick up all of my clothes so that inquiring canines don't discover the nethergarments that dogs so famously fancy.
UGH.
But on to the more general, over arching reasons for my lack of motivation: namely 1.) TSB, 2.) projects for new niece, 3.) work being done in therapy, 4.) money.
Well, if last night is any indication, I have gotten really accustomed to TSB's presence. Not that I did not enjoy my alone time. I relish it. but I enjoy the company too.
2.) Projects for niece are almost complete in the short term, i.e. those to be delivered at the end of the week when I travel to Houston. I have not finished the penguin - did not even have the motivation to knit - because if I started knitting then it would be like I was accepting the fact that I was staying home and not going back to the lab. I even watched some TV with my coat still on. Sad, I know.
But realistically speaking, once I have finished the projects for the niece in the short term, I can go back to all the projects I had started before everyone I knew was pregnant - namely two dresses, a shirt, two sweaters, umpteen skirts, a kitchen apron in an old-timey style. There are so many projects on my table I am sure I am forgetting some. I miss doing things for me, selfish as that sounds, I like making nice things that I can then show off. F* it, I am selfish and vane. There, I admit it. It is just that the projects for me do not have time constraints. So there is no driving force other than my various yens for either knitting or sewing or what have you. Oh, pillow shams. There pillow shams in that pile as well. Just remembered.
3.) I have mentioned the therapy a couple of times, and I am not ready to discuss the why. But I just might talk about the what... The addressing of my various demons requires time. Time to recover after my Monday sessions. Turns out I am uber over achiever and I would just power through and not acknowledge my feelings of discomfort or anxiety. And that was adversely affecting my work. So, with much urging from my peoples, I *give* my self permission to not go back to work on Monday nights after my meetings. I have given my self permission to knit, or shop, or go to a movie, or just drive around for hours. Things that don't require higher level cognitive function and allow my brain and psyche to refocus. But I feel guilty about taking the afternoon off, especially since I haven't been working weekends lately. Grad students don't take this much time off.
Sidebar - The German Nicaraguan and I used to make fun of our labmate, UConn, while he was planning his wedding last year. Mostly because he was working so little and focusing so much on his wedding. In retrospect, his quality of life was good, he kept his now-wife happy and the wedding was superb. But we were superior, snarky, competitive grad students making these comments. Thank God TGN has graduated and is not here to see how little I am working and how distracted I have been lately.
Also, this whole therapy thing requires a journal, something I have been keeping intermittently (that takes time). Then I stress when I don't keep it (that takes time). Then I stress ahead of my session each week, thinking that I have been a bad patient, not keeping enough of the journal (that eats even more time). Arg - it is a vicious cycle.
4.) Money - well this is a perpetual issue especially in my grad school years. I don't see it going away anytime soon. It just requires bandwidth in my thought processes, and that eats up time and motivation.
5.) Undergrads in the lab and general lab duties/ responsibilities - I just thought of this one. It turns out that at one of the senior lab members I get asked a lot of questions. Apparently, I am in the know. Who knew? Anyway, the nature of my boss' management style is hands off. Which works well for me, since by and large I can plan my own experiments, can order my own chemicals, can figure out software and computer programs, or at least die surfing the web trying to find resources. In short, I am generally self sufficient.
This is not true of the newer lab members, namely the undergrads and one of the postdoc.
When I was being trained as an undergrad, I was *given* to the lab manager, who made sure I was proficient on instruments and with equipment and techniques before letting me run wild. But we have no lab manager here, and the young'uns are allowed to run free, with little to no supervision until they screw something up. Then I have to go in and fix it. Some days I wear my headphones but don't have anything on, just so they will leave me alone.
The new postdoc is different - she has experience asking for help - and sometimes (more often than not) asks for help before even trying to figure something out. Which means that on more than one occasion I have had to explain something really really basic. This breeds contempt on my part. It indicates to me that she is either lazy or timid - both cardinal sins in my book. I think it is more timid, though.
Yes, laziness is a cardinal sin, thus my current mood where I am down on myself since I myself am lazy lately.
Yesterday, one of the undergrads asked for help on the diode, which is my baby so I am obligated not only as senior grad student but also as keeper of the instrument to make sure he is trained. I stopped my procrastinating about my notebook and started to instruct on the diode and software. While we are doing this, he starts asking some basic questions that really should have been asked weeks ago. These are questions that are answered by experience but the answers meant that everything he had done two weeks ago were for naught. I felt bad, it is a shame no one thought to clue this kid in. It would have saved him some work. It would have saved me some work, too, because after I told him that it was all crap, he asked me how to get it not to be crap anymore. DNA has a 1/2 life of like 3.4 billion years - but cells die and E. coli don't last for more than a night or two in solution at 4 C. And minipreps don't always give you the yields they promise, especially if you are doing them for the first time. So, dude, you are stuck with three week old dead liquid cultures, infinitesimally small amounts of DNA for cloning and sequencing and you tossed your plates. Basically, fucked. If I were you, here is what I would do... but I am not you, I do not know your project, your goals, where you have been and where your next focus should be. Sorry.
Wow, just had a fabulous conversation with a rotation student about - of all things - face cream. It is so nice to have a girl around here again. Yeah, boobs!
ciao